FastQCFastQC Report
Sun 14 Apr 2019
SRR6576139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217141
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23350.19184301572291132No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21790.1790260947581258No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19930.16374438130011232No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16200.13309879463431107No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15260.12537577815553003No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13650.11214805844187321No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13500.11091566219525921No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13430.11034054394683936No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13000.10680767470654592No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12210.1003170544743789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22400.059.811941
GGTATCA7850.055.693481
TCAACGC31200.042.3287774
ATCAACG32000.041.2705573
CAACGCA32850.040.2026755
AACGCAG33900.039.09616
TATCAAC37250.035.8412482
ACGCAGA41450.031.7480837
GTACATG54500.030.7937621
CGCAGAG43700.030.1134558
TACATGG55950.029.5756032
ACATGGG56800.028.960343
ACCTAAG7050.028.672821
GCAGAGT47300.028.119619
GTCTTAG7450.027.1333391
GGTAATC2601.8189894E-1225.3068858
CGTAGCT1301.0881658E-525.3068858
GTGTAGG4300.025.144931
CTAAGAC9550.025.0986753
AGAGTAC42900.024.7043410-11