FastQCFastQC Report
Sun 14 Apr 2019
SRR6576140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098321
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21180.19283979820107236No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20540.1870127221458936No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19540.17790791580967677No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15130.13775571986696056No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13130.11954610719452693No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12400.11289959856908864No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12300.11198911793546695No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11840.10780090702080722No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11690.10643518607037468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.060.3322751
GTATCAA19200.055.8161241
TCAACGC24550.041.7246444
ATCAACG24750.041.3874743
CAACGCA25950.039.654675
AACGCAG27000.038.112556
GTCTTAG7400.034.93471
CTTAGGT5700.032.9740833
TATCAAC32300.031.8703942
ACGCAGA33550.030.6717977
GTACATG45650.030.374371
TTAGGTA5950.030.009194
AGGTATA5950.030.009196
TACATGG46150.029.6392422
ACCTAAG7650.029.492111
TAGGTAT6300.029.0878525
CGCAGAG36200.028.4264858
ACATGGG48450.028.1249543
TCTTAGG8650.027.7140452
GGTATAG6750.026.4525457