FastQCFastQC Report
Sun 14 Apr 2019
SRR6576145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017590
Sequences flagged as poor quality0
Sequence length100
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17880.17570927387258128No Hit
GCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCC15110.14848809441916686No Hit
GTACATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC14850.14593303786397271No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT12650.12431332855079157No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12430.12215135761947345No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11970.11763087294489921No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11500.11301211686435599No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11200.11006397468528582No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10960.10770546094202971No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10710.10524867579280457No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10690.10505213298086656No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG10420.10239880501970343No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10180.1000402912764473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20350.064.9102551
GGTATCA6850.052.154821
ATCAACG29000.046.5125273
TCAACGC29450.046.4401664
CAACGCA30500.045.1495935
AACGCAG32000.043.1800776
TATCAAC37700.035.661212
ACGCAGA40400.033.7367067
CGCAGAG40850.033.4801188
GCAGAGT45050.030.5674259
TAGGTAT1751.70985E-1029.5420555
CCGTCCA6700.028.760419
GTACATG50300.028.0365561
TACATGG51500.027.5658362
GTATAGA1555.073889E-827.2949471
ACATGGG52700.026.754493
GGGTACC10050.026.6559937
ACCGTCC7250.026.5785878
GGTACCT10000.026.3192848
GTCTTAG4100.025.2237831
Produced by FastQC (version 0.11.2)