FastQCFastQC Report
Sun 14 Apr 2019
SRR6576145_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576145_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017590
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20160.19811515443351446No Hit
GTACATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC15140.14878290863707386No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT14730.14475378099234465No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14610.1435745241207166No Hit
GCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCC14190.13944712507001836No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13830.1359093544551342No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12850.1262787566701717No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12850.1262787566701717No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12240.12028420090606236No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12100.1189084012224963No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11680.11478100217179807No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11370.11173458858675892No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10630.10446250454505253No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC10590.1040694189211765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22800.060.3695951
GGTATCA8350.059.2752041
ATCAACG34500.039.7787933
TCAACGC34900.039.4575424
CAACGCA35700.038.7049945
AACGCAG37050.036.7872776
TATCAAC41500.033.975122
ACGCAGA45550.030.1273567
CGCAGAG45500.029.9538828
GCAGAGT48650.028.0144239
GTACATG54550.027.2198681
TACATGG55800.026.7843322
GTCTTAG4250.026.619051
ACATGGG55600.026.2044333
CTTAGGT2208.731149E-1125.6357753
ACCGTCC7800.024.7033468
AGAGTAC45400.024.5852310-11
GCGCGAC5950.024.4856289
ATGGGAG9800.024.4586225
TAGGTAT2501.6370905E-1124.4394385
Produced by FastQC (version 0.11.2)