FastQCFastQC Report
Sun 14 Apr 2019
SRR6576146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831611
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16370.19684684305522654No Hit
GTACATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC13600.16353800033910085No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT11630.13984903999586346No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10200.12265350025432564No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10140.12193200907635901No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10070.1210902693687313No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9510.1143563517077095No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9300.11183113258482631No Hit
GCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCC9300.11183113258482631No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9220.11086914434753749No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9030.10858442228397652No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8960.1077426825763488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17350.060.673711
GGTATCA6000.058.74381
TCAACGC25950.040.9208644
ATCAACG25950.040.7397963
CAACGCA26300.040.376285
AACGCAG26850.039.3742146
TAGGTAT1950.036.143515
TATCAAC30500.034.6684722
GTCTAGA1256.2700565E-933.8364261
CTATATG600.004162476431.3300251
TTAGGTA1807.2759576E-1231.3243734
ACGCAGA35000.030.6083897
CGCAGAG35450.030.2198498
GTACATG45050.029.1045841
GCGCGAC6550.028.6940849
ATGGGCG7600.028.4392365
AGGTATA2151.8189894E-1228.4104796
ACATGGG45850.028.2841683
TACATGG46000.028.094862
GCAGAGT38150.027.8347649
Produced by FastQC (version 0.11.2)