FastQCFastQC Report
Sun 14 Apr 2019
SRR6576147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344127
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8990.2612407628578984No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4510.13105626701769987No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4420.12844095348519588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.11420202425267416No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3840.11158671072017017No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3710.1078090356176644No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3700.10751844522516396No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3540.10286899894515689No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT502.9874112E-547.046691
CTAGTAC608.812481E-539.16573
GTATCAA9050.038.9889681
CGAACTC550.002710838334.190912
TACATGG17750.033.107392
GGGTACC3300.032.7567677
GTACATG18100.032.4908071
ACATGGG18250.031.9334563
TCAACGC10800.031.7677354
TGGGTAC3700.031.7559726
CAACGCA10850.031.6213385
AACGCAG11050.031.4743376
ATGGGTA3450.031.3325585
GGTACCT3450.031.3325588
ATCAACG11000.031.1901383
GTACTAG650.006118498328.951811
CATGGGT4900.028.77484
TAGGGTG1154.1859766E-628.6079885
CTAAATA1005.313746E-528.1993054
CATGGGG9450.027.353824