FastQCFastQC Report
Sun 14 Apr 2019
SRR6576148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723312
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20130.27830313889441904No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15280.21125047005994646No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15060.20820890570044465No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14670.20281704160860042No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10450.14447430707633774No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8920.12332160948525671No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8350.11544119273563828No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7820.10811378768774747No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7800.10783728183688367No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7670.10603999380626894No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7240.10009511801269715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19000.043.048851
GTACATG37900.033.7361561
ATCAACG23050.033.6425443
TCAACGC23250.033.353154
TACATGG37850.033.2839472
AACGCAG23450.033.26916
CAACGCA23650.032.7890325
GTCTTAG7550.032.3759351
GGTATAG6600.032.0438277
ACATGGG39250.031.8506153
TTAGGTA6500.031.813774
CTTAGGT6600.031.3317433
TAGGTAT6700.030.8641055
TCTTAGG8050.029.7810612
CATGGGG19300.029.7083384
GTATTAC953.746366E-529.688861
GTATAGT7550.029.2567948
AGGTATA7250.029.1709336
GTGCAAG3250.028.9276081
TATCAAC27850.028.5251142