FastQCFastQC Report
Sun 14 Apr 2019
SRR6576148_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576148_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723312
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20160.2787178976707147No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14790.20447607671378326No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14260.19714867166589245No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14010.1936923485300949No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10130.14005021346251686No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8780.12138606852921008No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8330.11516468688477446No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7840.10839029353861128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.10092463556528858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20950.041.856381
AACGCAG24200.034.7654086
ATCAACG24600.034.0090563
TCAACGC24650.033.940074
CAACGCA24800.033.734795
GTACATG39150.031.9114741
TACATGG39250.031.8530962
TAGGTAT5200.031.6354795
GTATAGT6250.031.5826788
ACATGGG39950.030.706723
TTAGGTA5450.030.1843074
GTCTTAG6250.030.17261
TATCAAC29400.029.2558982
TAAGTAT3100.028.8072365
ACGCAGA29050.028.7974157
CATGGGG21300.028.4655634
TATAGTA6950.028.4016889
CGCAGAG29600.028.262338
TTAAGTA2500.028.2007684
TCTTAGG6750.027.852612