FastQCFastQC Report
Sun 14 Apr 2019
SRR6576149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649041
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19770.30460325310727676No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14670.22602578265471673No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14580.22463912141143624No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14420.22217394586782654No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9570.14744831220215673No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8950.13789575697066903No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8450.13019208339688865No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7520.11586325054965711No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7490.11540103013523029No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7370.113552148477523No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6540.10076405034504753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18600.037.3964421
ACATACG651.4150474E-436.144138
TACATGG38450.034.3472062
GTACATG39350.033.6810651
ACATGGG39550.032.9089783
TAGGTAT4150.032.8345455
AACGCAG22200.031.748226
CTTAGGT4200.031.324913
TCAACGC22600.030.562584
GGTATAG4500.030.2807467
CAACGCA23000.030.0310555
ATCAACG23000.030.0310553
TCTTAGG5850.028.9219862
GTCTTAG4900.028.7744261
CATGGGG19900.028.5702064
GTATAGT5800.028.3544458
TGGGTAC5700.028.027556
TTAGGTA4700.027.9924764
AGGTATA4900.027.8088476
GGGTACC5650.027.4439497