FastQCFastQC Report
Sun 14 Apr 2019
SRR6576149_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576149_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649041
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19630.30244622450661823No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14640.2255635622402899No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13990.2155487865943754No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13800.21262139063633884No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9350.14405869582969336No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9180.14143944681460802No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8500.13096245075426668No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7030.10831365044735232No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.10507810754636455No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6780.10446181366046213No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6730.1036914463030841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17800.038.628391
GTACATG33850.036.4515841
TACATGG34100.036.2440952
ACATGGG34750.035.1604543
AGGGTAA2200.034.1769456
TCAACGC19800.033.4649244
AACGCAG19950.033.448866
ATCAACG19900.033.0606083
CAACGCA20100.032.9654465
CATGGGG18150.030.29324
TAGGTAT4850.029.068025
AAGGGTA2450.028.7714085
ATAGTAC1154.197349E-628.6046163
TTAGGTA5250.027.7484234
GTCTTAG5950.027.7028751
GGTACCT5100.027.6580338
GTATAGT6300.027.614138
ACGCAGA24400.027.3485587
CTTAGGT5200.027.1115173
TATCAAC24800.027.0969432