FastQCFastQC Report
Sun 14 Apr 2019
SRR6576150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468753
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7370.15722566042243996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.11861257421285837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13500.039.705161
TACATGG26900.033.385422
GTAATAG851.7527796E-533.1759073
GTACATG27250.032.7840731
ACATGGG28050.031.5003553
AACGCAG16950.031.33286
ATCAACG17700.030.005143
GTACTAG953.7371217E-529.6963751
TCAACGC17750.029.6558344
CAACGCA18150.029.261215
CATGGGA14300.027.6079224
ACGCAGA19500.027.2354347
TATCAAC19650.027.0390682
CGCAGAG19650.026.788358
GTACACT1807.4742275E-926.1106664
ACAATAG1458.633433E-725.9305923
ATGGGAG5700.025.5609665
CATGGGT5550.025.4049744
TAATAGT1351.4552417E-524.3699534
ATGGGTA3550.023.830585
Produced by FastQC (version 0.11.2)