FastQCFastQC Report
Sun 14 Apr 2019
SRR6576150_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576150_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468753
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6330.1350391357495312No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.11797257830883216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.044.589931
ATCAACG15600.036.151923
AACGCAG16400.035.8212626
TCAACGC15750.035.5092164
CAACGCA16300.034.599385
GTACATG25450.031.8645151
TACATGG25250.031.6418782
GTATTAC1351.2836608E-831.4322451
TATCAAC18400.030.905962
ACGCAGA19050.030.5915457
CGCAGAG19100.030.2654028
ACATGGG26850.029.7563273
TATCGTA700.00883307826.8557117
CTCACGT459.5193274E-426.10971830-31
GCAGAGT22000.026.0622469
CATGGGT6500.025.3063434
ACCTATA1201.7969732E-423.5741841
CATGGGG11550.023.1935674
ATGGGCG1652.6594844E-622.7866655
CATGGGA13100.022.6018474
Produced by FastQC (version 0.11.2)