FastQCFastQC Report
Sun 14 Apr 2019
SRR6576151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422549
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6970.16495128375644008No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.12945244220196947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA352.938065E-453.7043464
CGCTAAA405.677798E-446.9913025
GTATAAC550.002715960834.179541
CCCGCTA550.002717537834.175493
GTATCAA13250.032.9864771
ATATAAC1001.4104044E-632.893913
ATGGGTA3500.030.885
GTACATG26100.028.8103371
GGTACCT3700.027.9374688
TACATGG27100.027.4003852
CATGGGT5500.027.3403954
CCTATAC1406.36257E-726.8521733
ACATGGG27100.026.7035433
AACGCAG16200.026.6864196
ATCAACG16250.026.6043053
CAACGCA16500.026.201215
TCAACGC16700.026.1688084
GGGTACC4000.025.8421557
TGGGTAC3800.024.7322656
TATCAAC18150.024.3399732
Produced by FastQC (version 0.11.2)