FastQCFastQC Report
Sun 14 Apr 2019
SRR6576151_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576151_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422549
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6510.15406497234640243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5850.13844548206243537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4600.10886311410037651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAA555.2802658E-542.7233432
ACTCCGC450.001011325641.793728
GTATCAA12500.038.0355341
GTACATG25000.033.139871
TACATGG25000.032.8969732
ACATGGG24950.032.2094653
ATCAACG15450.030.7220953
TCAACGC15700.030.232894
CAACGCA15850.029.9467745
TAATACT2200.029.9063424
AACGCAG16000.029.6660236
CATGGGA13250.026.601334
TATCAAC18100.026.4837512
ATGGGAG5100.025.8015525
ACGCAGA18700.025.1284077
ATAATAC1701.2410783E-724.8800663
TTAGTAC950.001303919124.7345683
CATGGGG9750.024.5823554
CGCAGAG18900.024.3796758
CATGGGT5400.022.627554
Produced by FastQC (version 0.11.2)