FastQCFastQC Report
Sun 14 Apr 2019
SRR6576152_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576152_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147469
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4210.28548372878367656No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3920.26581857881995535No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2790.18919230482338661No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2680.18173311000956133No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2300.1559649824708922No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2150.14579335317931225No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2140.1451152445598736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.14104659284324164No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2010.136299832507171No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2010.136299832507171No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1990.13494361526829368No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1940.13155307217110038No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1820.12341576873783644No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1810.12273766011839776No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1810.12273766011839776No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1690.11460035668513383No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1650.11188792220737918No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1610.10917548772962453No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1590.1078192704907472No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1560.10578494463243121No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1510.10239440153523792No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG1500.10171629291579926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAG250.0051785856.5327641
GTATCAA5650.043.3584631
GTACATG9750.038.17171
GGTATCA2500.037.688511
TCAACGC6350.037.006944
ACATGGG9800.036.927643
AACGCAG6400.036.7178236
ATCAACG6450.036.433193
CCTAAGA651.407733E-436.1529352
CTCTATA651.407733E-436.1529352
CAACGCA6800.035.249115
TACATGG10700.034.2608152
CTAAGAC550.002709705434.1809543
TAGGCAT550.002709705434.1809545
ACCTAAG702.1459506E-433.6504551
ATGGGAT1405.0386006E-1033.570585
GTATAGT851.7166063E-533.254571
CATGGGG3300.032.756754
CGCAGAG7550.031.7475438
CATGGGT2700.031.3325444
Produced by FastQC (version 0.11.2)