FastQCFastQC Report
Sun 14 Apr 2019
SRR6576153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140800
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4160.29545454545454547No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3080.21875000000000003No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2990.21235795454545453No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2350.1669034090909091No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2240.1590909090909091No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2160.1534090909090909No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1990.14133522727272727No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1940.1377840909090909No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1920.13636363636363638No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1900.13494318181818182No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1890.13423295454545453No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1860.13210227272727273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.13068181818181818No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1820.12926136363636365No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1800.1278409090909091No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1770.1257102272727273No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1720.12215909090909091No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1620.11505681818181819No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1550.11008522727272727No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1530.10866477272727273No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT1510.10724431818181818No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTGCTCATCTCGTATGCCGT1500.1065340909090909No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1490.10582386363636365No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1490.10582386363636365No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1430.1015625No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG1420.10085227272727272No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG1410.10014204545454544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTC156.887252E-494.007812
CAAACGC603.583409E-1062.6718752
GCCCGCA301.3664416E-462.6718752
TCTAGAC301.3688463E-462.649623
AAACGCA657.3123374E-1057.830423
TGCCCGC504.5321758E-756.4046861
CCGAACT250.005224807756.4046861
CTAGACC250.00523216156.384664
CCGCAAA352.9325846E-453.6996734
CCCGCAA352.9325846E-453.6996733
GCAAACG954.2018655E-1044.5300181
TAAGGTG450.001012277741.7664155
CCGGTCC450.001012277741.7664159
CGCCGGT450.001012277741.7664157
TGACAAG250.001702119437.5897716-17
GGGGGAA500.001699229637.589777
ATAGACG651.4094546E-436.1440128
GTACATG11350.034.7870331
ACATGGG11450.034.4709743
TCTTGCG550.002707848834.184662
Produced by FastQC (version 0.11.2)