FastQCFastQC Report
Sun 14 Apr 2019
SRR6576153_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576153_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140800
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7260.515625No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3820.2713068181818182No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2580.18323863636363638No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2520.17897727272727273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.16619318181818182No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2180.15482954545454544No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2160.1534090909090909No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2150.15269886363636365No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2020.1434659090909091No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1880.13352272727272727No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1780.12642045454545453No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1740.12357954545454544No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1730.12286931818181819No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1690.12002840909090909No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1600.11363636363636363No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1590.11292613636363635No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1570.11150568181818182No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1510.10724431818181818No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1510.10724431818181818No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1480.10511363636363637No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1440.10227272727272728No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1420.10085227272727272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAT200.002161262370.481623
TGCGAGT250.0052025656.46550494
CATACTG250.005231925356.3852965
GGTACCT1300.050.638168
GGGTACC1300.050.6021927
GTATAGG405.6076323E-447.0880781
TGGGTAC1400.043.631486
ATGGGTA1550.039.4090775
GTATCAA5850.038.6363751
ATGACAG500.001699134837.59025
GTACATG9650.034.6451151
AACGCAG6900.033.3681146
GTACCTG2000.032.9148039
ACATGGG10000.032.8914223
TACATGG10300.032.3896142
ATCAACG7150.031.5442243
CCTGGGT600.00415400231.3251693
TCAACGC7450.030.9046924
GGTATCA3050.030.877431
CATGGGT2750.030.7556174
Produced by FastQC (version 0.11.2)