FastQCFastQC Report
Sun 14 Apr 2019
SRR6576154_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576154_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321859
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.14291972571840464No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3800.11806412124563861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGT250.00523334456.3980459
GTATCAA10050.044.537021
TAACGCC608.8036606E-539.1713944
ATTAGAC500.001699199837.6045383
AACGCCG757.4207783E-637.6045385
GTACATG18500.036.673671
ATCAACG12150.036.3665283
TACATGG18650.035.7898452
AACGCAG12350.035.7775966
CAACGCA12400.035.633335
ACATGGG18500.035.3177723
TCAACGC12850.034.385474
CGCAGAG13650.032.3651778
ACGCAGA13900.032.121197
CATGGGG7800.031.9397534
GTAGTAT753.2236025E-431.4103221
TATCAAC14600.030.585882
ATAACGC804.7699537E-429.3785463
ATGGGAG4200.029.098755
GGTATCA5050.028.9223751
Produced by FastQC (version 0.11.2)