FastQCFastQC Report
Sun 14 Apr 2019
SRR6576156_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576156_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358215
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5300.14795583657859107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.10859400080956967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11850.035.4009931
GTACTAC650.00606299629.006171
AACGCAG15650.028.5284466
CAACGCA15500.027.5917035
GTACATG22050.027.5755961
ACATGGG21850.027.531343
ATCAACG15500.027.2884983
TCAACGC15600.027.1135734
TACATGG22450.026.7955362
GTAATAC909.5085544E-426.1093673
TATCAAC17050.024.8077282
CGCAGAG18550.024.3218278
CATGGGA13750.024.267474
ACGCAGA19000.023.745787
GTATATC1252.372381E-422.6248151
TCCCGAC1252.4200119E-422.5584932
GTATTAC1050.00229381822.4452511
TAATACC1050.00233328822.3794574
TAGACTC1050.00233328822.3794575
AGTACTT8600.022.13224212-13
Produced by FastQC (version 0.11.2)