FastQCFastQC Report
Sun 14 Apr 2019
SRR6576157_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576157_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325408
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5130.15764824466515884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.1536532599075622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG250.001698056837.6154472-73
GTATAGA1055.016409E-835.8711131
GTATCAA10800.034.874691
TCTATAC959.4210736E-734.6324843
TACCGTC959.432424E-734.6271637
ATACCGT1151.13279384E-732.6965076
CATGGGT4900.029.7354724
ACCGTCG953.7277492E-529.7032478
GTACATG20300.029.6864341
TATACAG1901.4551915E-1129.6849865
TATACCC804.7727054E-429.375774
TACATGG20350.029.332462
ACATGGG20900.028.1107853
AACGCAG13850.027.4880836
ATCAACG13700.027.4459743
CAACGCA14200.026.810565
TCAACGC14350.026.2027764
TATCAAC15050.025.9209422
TAATACT1101.01595855E-425.6370334
GTATAAT1859.798896E-925.4490991
Produced by FastQC (version 0.11.2)