FastQCFastQC Report
Sun 14 Apr 2019
SRR6576158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469168
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8630.18394263888415238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.1317225386215599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11400.045.7950941
ATCAACG14300.035.4947743
TCAACGC14750.034.411884
TATCTCG550.00271602734.180155
CAACGCA15200.033.393115
AACGCAG15700.032.3296366
GTACATG24950.031.669381
ACATGGG25500.030.778893
CATGGGG9400.030.4985124
TACATGG25700.030.3597322
GTCTTAG1751.6916601E-1029.563471
TATCAAC17700.028.9451772
GGTACCT3800.028.4459828
ATGGGTA4200.027.9748275
ACGCAGA18150.027.965587
GGGTACC4150.027.1793987
CATGGGT6750.027.1542324
CGCAGAG18700.027.1430638
CGCTATA350.00883501226.85726746-47
TGGGTAC4300.025.138316
Produced by FastQC (version 0.11.2)