FastQCFastQC Report
Sun 14 Apr 2019
SRR6576159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519113
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10380.19995646419950958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.11327013578931755No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5290.1019045949533146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10350.044.077711
ATCAACG13250.033.697873
GTATTAC851.7449487E-533.1986161
TCAACGC13500.033.0738334
CAACGCA13550.032.9517945
TAGCCCG753.273224E-431.3331095
ACATGGG23150.031.0624123
GTACATG23950.031.0269621
AACGCAG14450.030.5741756
TACATGG23750.030.2776762
CTAGTAC1452.6544512E-829.1722033
CCGAACT1005.2938547E-528.2188241
CATGGGT6700.026.6565274
ACGCAGA16650.026.5343447
GTATAGG1458.5850115E-725.9483431
TATCAAC17250.025.8838732
CATGGGG12650.025.6361794
CGCAGAG17500.025.5141038
GTACTAG1301.0807213E-525.3245851
GTATTAT1901.3198587E-824.7533551
Produced by FastQC (version 0.11.2)