FastQCFastQC Report
Sun 14 Apr 2019
SRR6576160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199784
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6690.33486165058262923No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4320.216233532214792No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3850.19270812477475674No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3480.17418812317302687No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3410.1706843390862131No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3150.15767028390661914No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3110.155668121571297No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2870.14365514755936412No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2770.13864974172105873No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2770.13864974172105873No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2660.13314379529892284No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2610.13064109237977015No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2520.12613622712529532No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2500.12513514595763425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.12063028070315941No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2400.12012974011932886No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2320.11612541544868459No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2250.11262163136187082No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG2220.11112000961037921No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2160.108116766107396No Hit
GAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA2150.10761622552356545No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2110.1056140631882433No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2080.10411244143675169No Hit
GCATTTATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCC2060.10311136026909062No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG2060.10311136026909062No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2050.10261081968526008No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2010.10060865734993793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA2100.035.807715
GTACTTG801.1522905E-535.2570421
GGGTACC1900.034.6298267
TGGGTAC2050.034.3885046
GTACAAG550.002709006734.188651
GGTTAGG550.002709006734.188651
CATGGGT3400.033.174794
GTATCAA9500.031.6694831
TCTTAAT902.5797668E-531.3395922
TACATGG15400.030.8308332
ACATGGG14950.030.8078043
GTACATG15650.030.3383271
TCAATAC953.7382364E-529.6827093
CCTTTAC650.00613617328.9288562
AATAGCA650.00614368628.9216165
ATGGGAG2300.028.6072485
GTACAGC856.7883154E-427.652581
GCGTTAT856.7883154E-427.652581
CTGTGCG856.798263E-427.6456589
CATGGGC2650.026.6024274
Produced by FastQC (version 0.11.2)