FastQCFastQC Report
Sun 14 Apr 2019
SRR6576162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444399
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6490.1460399325831066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.12353763172284367No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5180.11656191845616215No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4620.10396062997441488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10150.050.4756241
ATCAACG13300.037.103093
TCAACGC13650.036.1517264
TACATGG24250.035.6637762
GTACATG24450.035.5642851
CAACGCA14000.035.2479325
AACGCAG14300.034.8371166
ACATGGG26100.033.1321563
CATGGGT6500.032.5365524
ACAACGC600.004159454331.3314973
GTCCTAG1859.094947E-1230.4881291
TATCAAC16850.029.8473052
CGCAGAG17300.028.5243388
ACGCAGA17550.028.38587
CATGGGA13400.027.7073294
GTATAAT2450.026.858591
GGTACCT3200.026.435958
GGTATCA4400.025.6377471
GCAGAGT20150.024.4898789
CGACGGT1000.001757589223.4986217
Produced by FastQC (version 0.11.2)