FastQCFastQC Report
Sun 14 Apr 2019
SRR6576162_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576162_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444399
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.1496403007207487No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6410.1442397485142856No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4930.1109363432410964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10700.048.042821
ATCAACG14550.035.8572583
TCAACGC15150.034.7474144
CAACGCA15350.033.6822745
GTACATG25700.033.3982731
TACATGG25500.033.177942
AACGCAG15600.033.1424946
ACATGGG26050.032.1165853
GTTCGGG600.004089101731.4408661
TATCAAC17100.030.2352582
GTATAAG1303.225614E-729.0223431
ACGCAGA18050.028.3803357
CATGGGA12900.027.6911494
ATATAGT1205.830605E-627.417886
CATGGGG10650.026.9213814
CGCAGAG19050.026.8905538
GGTATCA4950.026.6771011
ATAGACT1956.020855E-1026.5139944
CATGGGT5500.024.7829174
ATGGGTA3700.024.1362045
Produced by FastQC (version 0.11.2)