FastQCFastQC Report
Sun 14 Apr 2019
SRR6576163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410055
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7260.1770494201997293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.13998122203119093No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5030.12266647157088684No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4850.11827681652461254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA301.3713723E-462.6550374
GTATCAA10850.047.6525541
TACTAGC500.001700262337.6021962
ATCAACG13700.037.044223
AACGCAG13600.036.971086
TCAACGC13900.036.511214
CAACGCA13950.036.3803445
ACATGGG22500.035.9222223
TACATGG23100.035.608142
GTACATG23050.035.277551
GTATACT702.1849407E-433.56524
GTACTAG600.004156731.3351631
AGTATAC600.00416166631.3275183
TATCAAC16700.030.9598922
ACGCAGA16450.030.562037
CGCAGAG16950.029.9376968
GTAATAG1102.96096E-629.9108371
GTAGGAC953.7512007E-529.6787053
GGTCTAT650.00614785928.9247671
ATACTAA650.006155191528.9177116
Produced by FastQC (version 0.11.2)