FastQCFastQC Report
Sun 14 Apr 2019
SRR6576164_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576164_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432182
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6850.15849804017751778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5640.13050057614616065No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4570.10574248811843158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7950.058.0558741
GGTATCA3200.041.2093051
ATCAACG11700.038.567143
TCAACGC11850.038.078954
CAACGCA12200.036.6012465
AACGCAG12700.035.160256
GTACATG22250.034.9253851
TACATGG22650.034.241112
ACATGGG23000.033.9244083
TATCAAC13750.033.500822
ACGCAGA15200.029.3739157
CGCAGAG15850.028.1693088
CATGGGG10150.027.785444
CATGGGA11400.027.212674
ATGGGTA3200.026.4395855
GCAGAGT17350.025.733929
ATGGGGA6550.024.3988695
GGTACCT3100.024.2571688
GATATAA1401.8973915E-523.5481741
CATGGGT5750.022.8887584
Produced by FastQC (version 0.11.2)