FastQCFastQC Report
Sun 14 Apr 2019
SRR6576165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400850
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6500.16215541973306724No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5140.12822751652737932No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.1279780466508669No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4150.10352999875265062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10150.054.6328541
ATCAACG13700.039.7851643
TCAACGC14150.038.5199134
CAACGCA14550.037.7838825
AACGCAG14550.037.7838826
TATCAAC16400.033.8124052
GTATTAC851.7535995E-533.171891
GTACATG23000.031.6695421
TACATGG23300.031.4634672
CGCAGAG17950.030.6270478
ACGCAGA18150.030.2895587
ACATGGG24000.029.9546263
GTGCTAT804.7792523E-429.3709471
TACTATA1354.6276E-727.8480072
AGTCCGC700.00884060426.8500868
GCAGAGT20850.026.3671729
ATGGGGG3100.025.767425
CATGGGG10350.025.4232714
CATGGGA11500.024.5152954
CATGGGT4800.024.4727364
Produced by FastQC (version 0.11.2)