FastQCFastQC Report
Sun 14 Apr 2019
SRR6576166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621705
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13750.046.8524631
AACGCAG17050.036.6604046
ATCAACG17300.035.858973
TCAACGC17700.035.314124
CAACGCA18000.034.4644555
TACATGG31850.033.6620062
TATATCG702.1846757E-433.5692755
GTACATG32050.033.158511
ACATGGG32750.031.8574983
TATCAAC19850.031.7437952
ACGCAGA20300.030.5596167
CGCAGAG22300.027.8188428
CATGGGG18150.024.8579084
CATGGGA14450.024.3928994
ATGGGTA3350.023.8492155
GCAGAGT26150.023.7231459
TATAGTG1852.812194E-722.8633985
TCGAACG1703.4495315E-622.1162283
GGTACCT3007.2759576E-1221.9319278
GTAATAC1503.2898693E-521.9319273
Produced by FastQC (version 0.11.2)