FastQCFastQC Report
Sun 14 Apr 2019
SRR6576166_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576166_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621705
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7120.11452376931181188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15800.044.448671
CGCTAAA250.001702610837.5976224-25
CAACGCA19950.034.6293835
TCAACGC20300.034.2638444
AACGCAG20050.034.222276
ATCAACG20550.033.618313
TATCAAC22400.031.681032
GTACATG31700.031.521451
TACATGG32000.031.4292562
TTAGTAC902.5931447E-531.331353
ACATGGG32500.030.5119133
ACGCAGA23800.029.0275757
CGCAGAG25400.027.1990648
CATGGGG16800.026.8554424
CCACGAC1458.644529E-725.9293923
TAGCCCT2800.025.1769774
GCAGAGT28200.024.6651049
TGTATCG1151.3768065E-424.5201879
TATCGTG802.0401494E-623.49851410-11
CCGCTAA500.001759408623.4985124-25
Produced by FastQC (version 0.11.2)