FastQCFastQC Report
Sun 14 Apr 2019
SRR6576167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559581
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5930.10597214701714318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14950.043.710011
CCGAACT500.001699175637.6094781
AACGCAG17550.036.143576
ATCAACG18150.035.46653
TCAACGC18250.035.01474
GTACATG30250.034.6566661
ACATGGG29750.034.4305463
CAACGCA18650.034.2637185
TACATGG30650.034.2043762
TATCAAC19450.033.5971572
ACGCAGA20150.031.4798877
CATGGGG14250.029.6757744
CGCAGAG21400.029.6411048
AACGTAT350.0088481826.84950860-61
ATAACGC1406.374303E-726.8495083
ATGGGAG7200.026.7562835
CATGGGA14650.026.2996924
GCAGAGT24500.025.8905969
CTATACT1501.1636275E-625.0595424
GCTATAT1351.4528638E-524.3765141
Produced by FastQC (version 0.11.2)