FastQCFastQC Report
Sun 14 Apr 2019
SRR6576168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415794
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6400.1539223750222466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.1428592043175226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13600.041.479081
CTAGTAC757.4339823E-637.598653
TACATGG27750.033.880812
GTACATG28350.032.9979361
ACATGGG28700.031.9326533
ATGGGAG5600.031.8917165
TAGTACT1501.0659278E-931.332214
AACGCAG17750.030.4496146
ATCAACG18150.030.037493
CAACGCA18550.029.3897825
TTACGCG404.7706693E-429.3845580-81
TCAACGC18700.028.9027084
CATGGGG10800.028.7211954
TATCAAC19950.027.5695532
CCTAGTA1205.8221503E-627.4222812
CGACCGT350.00883618426.85618214-15
ACACGCC909.5094176E-426.1101766
CATGGGA14900.025.8648454
GTATAAG1101.0153913E-425.6416131
CGCAGAG21350.025.5353858
Produced by FastQC (version 0.11.2)