FastQCFastQC Report
Sun 14 Apr 2019
SRR6576168_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576168_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415794
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.1623400049062757No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5830.14021366349682776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG200.002162280370.497752
CCGTATG250.00523186856.40497694
GTATCAA14400.038.951461
CTAGTAC757.433784E-637.59883
CCTAGTA851.7524842E-533.1754072
GTACATG26250.031.7821481
TACATGG26500.031.0367432
ACATGGG27150.030.6399043
AACGCAG18550.030.4033416
CAACGCA19250.029.0536165
ACTCACG650.00615067428.9221528
ATCAACG19050.028.8652213
TCAACGC19750.028.0801164
CATGGGG8550.026.9348134
TATCAAC20600.026.693322
ATGGGGA4250.026.5403275
ATGGGAG6350.025.9046845
ACGCAGA21800.025.8707317
CGCAGAG21450.025.854658
GCCTAGT950.001281649924.8076551
Produced by FastQC (version 0.11.2)