FastQCFastQC Report
Sun 14 Apr 2019
SRR6576169_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576169_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383278
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.17350330569456113No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5550.14480351076764122No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4840.1262790976784475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.1004492822442196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16550.037.5397641
GACTTAT702.1641355E-433.6192171
ATGGGAG5500.030.7591975
AACGCAG20450.030.5628266
TACATGG29200.029.290292
ACATGGG29300.029.1903233
GTACATG29450.029.0871851
GTATAAT2151.8189894E-1228.4590571
ATCAACG21500.028.4145013
CAACGCA21950.028.2601575
TCAACGC22150.027.5806674
GGTACCT3100.027.3041958
ATGGGTA3450.027.2424455
ACGCAGA23000.027.174347
CATGGGT6500.026.7499854
TATCAAC23100.026.6498322
GTATAGG1606.796472E-826.4751321
CGCAGAG23650.026.4447258
TGGGTAC3200.026.4336836
TATAGGG1101.0176003E-425.6326622
Produced by FastQC (version 0.11.2)