FastQCFastQC Report
Sun 14 Apr 2019
SRR6576170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466028
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11500.2467662887208494No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6620.1420515505506107No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6220.13346837529075506No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5900.12660183508287057No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5820.12488520003089944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.11673118353403658No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5320.1141562309560799No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5000.10728969074819537No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4970.10664595260370621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14100.038.3593861
ATCCTAT1451.8189894E-1135.6794051
TAGACGG702.1839386E-433.5690165
ATCAACG16550.032.3722953
TGGGTAC4250.032.0682836
TCAACGC16750.031.985764
CCCTATA753.2739562E-431.3310812
CAACGCA17300.031.2405265
AACGCAG17650.030.6210276
GGGTACC4500.030.286717
GCTATAT804.778583E-429.3728873
CATGGGT7050.028.6646044
TACATGG26150.028.5753822
GTACATG26150.028.2371521
ATGGGTA4850.028.1010725
GGTACCT4900.027.8143258
TATCAAC19450.027.5455762
ACATGGG26950.027.2039813
ACGCAGA20100.026.8886157
CCCGCTA700.00883382526.8552113