FastQCFastQC Report
Sun 14 Apr 2019
SRR6576170_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576170_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466028
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10210.21908554850781498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.14741603508802045No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6500.139476597972654No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6370.13668706601320094No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5050.10836258765567736No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4770.1023543649737784No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4670.1002085711588145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13050.037.6023981
CTCGATA651.4119351E-436.15594
CTTATTA550.002712076534.190252
TTATTAC702.1839824E-433.56893
ATGGGAG3750.032.584215
TACATGG26600.031.1054122
GTACATG26900.030.8711721
ACATGGG26950.030.3427963
ATCAACG16500.029.6220113
CAACGCA17050.028.9421025
TCAACGC17050.028.6664624
AACGCAG17550.028.6531126
GGGTACC4650.028.2989437
TGGGTAC4850.028.1009756
GGTACCT4850.026.1629778
GTAGGAC909.5133414E-426.1091443
CATGGGG15350.025.10564
ATGGGTA4950.024.6850095
TAAGACT1151.3761962E-424.5198924
ATGGGGG5850.024.1007465
Produced by FastQC (version 0.11.2)