FastQCFastQC Report
Sun 14 Apr 2019
SRR6576171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235972
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2730.11569169223467192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.11103012221789026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC352.9327197E-453.7142839
GTATCAA7150.049.9580421
ATAAGAG757.418512E-637.6000023
ATCAACG9600.035.7395823
TCAACGC9650.035.55444
AACGCAG9700.035.371136
CAACGCA9850.034.8324855
CCGCCTA550.002712654634.1818169
TATCAAC10300.033.3106772
CTAATCC851.7489047E-533.176474
ACGCAGA10850.031.6221227
TGCGAGT600.004154343631.33333494
CGCAGAG11000.031.1909078
TATACAG1252.3497341E-730.085
CCCTTAG804.769508E-429.3751
ATAGACT650.00614439628.9230773
AATAGAG650.00614439628.9230773
TAGAGAG1005.3058742E-528.1999995
GTACATG14200.027.8028181
CATAGAC856.799931E-427.647062
Produced by FastQC (version 0.11.2)