FastQCFastQC Report
Sun 14 Apr 2019
SRR6576173_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576173_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488618
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11820.24190676561240068No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7910.1618851536374018No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7640.15635936457518962No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6880.14080529165933306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.1283211015558166No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6230.12750246613919258No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6180.12647917186841254No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5920.12115804166035636No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5740.11747418228554823No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5560.11379032291074008No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5150.10539930989034378No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4920.10069215624475562No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4910.1004874973905996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11400.053.7043651
AGGTATA1350.045.2621656
GTACATG26100.039.516151
AACGCAG14950.039.3001826
TACATGG26200.039.1133842
ATCAACG15150.039.095623
TCAACGC14950.038.9897734
CAACGCA15300.038.0939525
ACATGGG28250.036.441473
GTGTTAG801.1414497E-535.3209461
CTCGATA702.1836598E-433.5701594
ACGCAGA17850.032.911917
TATCAAC18650.032.2627452
GATATAT753.2344382E-431.3963951
GTCTTAG2900.030.855081
CGCAGAG18800.030.748838
CTTAGGT1859.094947E-1230.4915683
TAGGTAT1701.2187229E-1030.4137175
GGTACCT5100.030.4106068
CATGGGG16950.030.2292234
Produced by FastQC (version 0.11.2)