FastQCFastQC Report
Sun 14 Apr 2019
SRR6576174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440660
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12250.2779921027549585No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8060.1829074569963237No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7930.17995733672218944No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7540.17110697589978668No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7400.16792992329687287No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6150.13956338219942813No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5960.13525166795261653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.12821676576045024No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5090.11550855534879498No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4840.10983524712930605No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4830.10960831480052648No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4820.10938138247174693No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4750.10779285617029002No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4680.10620432986883312No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4470.10143875096446239No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4450.10098488630690328No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4450.10098488630690328No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4410.10007715699178504No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG4410.10007715699178504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT450.001014328241.768954
GTATCAA11450.041.4497571
TACATGG24650.034.317132
GTAATAC550.00271801334.1745953
GAACCGT550.00271801334.1745956
TATAAGA1405.0931703E-1033.5681462
GTACATG25400.033.485051
CTATAAG1001.4108755E-632.8930471
ACATGGG26250.032.042753
GTCCTAA600.00416252531.3267151
TACGCGC300.004164763731.32671536-37
GTTGTAT600.00416252531.3267151
CTACGAT600.00416252531.3267151
CGTACGA300.004164763731.32671558-59
CTTACGC300.004164763731.32671534-35
AACGCAG15450.030.4142866
TCAACGC15600.029.5194024
ATCAACG15900.028.9624353
CTATACA650.006156456728.9169674
GCCTATA650.006156456728.9169671
Produced by FastQC (version 0.11.2)