FastQCFastQC Report
Sun 14 Apr 2019
SRR6576175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97821
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.2504574682327925No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1330.13596262561208738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.13187352408991934No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1250.1277844225677513No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1160.11858394414287321No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1130.11551711800124717No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG1060.10836119033745309No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG1040.10631663957636908No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT1040.10631663957636908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT200.002157050470.51
GGACACC250.00522176956.3999986
CATGGGT1800.047.0000044
CATGGGG2100.044.7619064
ATGGGTA954.1836756E-1044.5263185
ATGGGAC954.1836756E-1044.5263185
CCTATTG751.4624675E-743.8666794
ATGGGGC652.758752E-643.3846175
GTATCAA6450.042.992251
ATGGGAT1600.041.1255
ACCCTTG802.427896E-741.1257
GTACATG10550.040.9857831
ACATGGG10400.040.6730773
TACATGG10650.040.600942
AACGCAG6800.039.3970576
TGGGATC608.764986E-539.1666686
CGCGAGG250.001698577237.676-77
AGGCGAG250.001698577237.680-81
AAGTTAA500.001694425537.694
CGAGGCG250.001698577237.678-79