FastQCFastQC Report
Sun 14 Apr 2019
SRR6576175_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576175_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97821
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.26885842508254876No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1490.15231903170075956No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1360.1390294517537134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.1380071763731714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.10938346571799512No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1000.10222753805420104No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA980.10018298729311702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG200.002135478770.678761
AGGTCCG250.005222330356.3984687
ATGGGTT555.2484265E-542.7261125
TACGCTA802.4284236E-741.1238829
CATGGGG2750.041.0170674
ACATGGG8850.038.7673073
CCTTGGC853.907262E-738.704839
TACATGG9300.037.9021952
GTACATG9200.037.9002041
ACCCTTG500.001694651637.5989767
ATACGCT906.1147693E-736.554568
GTATCAA5600.034.4979631
CTTACTA600.004143115631.3324819
CATGGGA4700.030.9991574
AACGCAG6100.030.8188346
CATGGGT2000.030.549174
TATACGC953.7183643E-529.6834037
ATCAACG6400.029.37423
CTATTAA1452.5444024E-829.2463821
CAATACT650.00612786728.9222894
Produced by FastQC (version 0.11.2)