FastQCFastQC Report
Sun 14 Apr 2019
SRR6576176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95200
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.2542016806722689No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1550.16281512605042017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.14600840336134452No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1230.12920168067226892No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1120.1176470588235294No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1070.11239495798319328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.11029411764705882No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT1030.10819327731092437No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1010.10609243697478991No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG990.10399159663865545No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA960.10084033613445378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT200.002159552470.477941
CATGGGG2450.044.1086464
GTATCAA6050.041.937291
TGGGGCA608.779395E-539.1544156
ACATGGG9100.037.175183
GTACATG9250.036.572341
TACATGG9500.036.104492
ATGGGGC801.1500049E-535.238975
ATGGGGT959.353098E-734.6207435
AACGCAG6800.034.548016
ATGGGAT1107.591552E-834.1711245
GAATAAC550.002708721234.1711241
CCCTGGT550.002708721234.1711247
GGGGCAG702.1740368E-433.5609257
TAATACC702.1740368E-433.5609254
ATGGGAC600.004148363631.323535
GGGCAGT600.004148363631.323538
ATCAACG7400.031.1118833
ACGCAGA7600.030.9113797
CAACGCA7450.030.9030785