FastQCFastQC Report
Sun 14 Apr 2019
SRR6576176_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576176_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95200
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.3161764705882353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2300.2415966386554622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.17857142857142858No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1370.1439075630252101No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1160.12184873949579833No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1090.11449579831932773No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG1060.11134453781512606No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA980.10294117647058824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATG352.9257542E-453.707087
TGGGTAT558.733314E-751.265856
TGTATCG450.001007327241.7941259
CATGGGT1800.041.7721754
ATGGGTA1156.002665E-1140.8640865
GTATCAA7750.034.635871
CGAGAAA550.00269242334.21317394
GTATGGC550.00269937834.195199
GGTATGG550.00269937834.195198
CCTACAG352.1731864E-433.58456468-69
TGGGGCA702.1717546E-433.5669256
GTACATG10350.033.215091
ACATGGG10100.032.569893
CATGGGG2600.032.5340964
ACGTGCC300.00414973431.3373666-67
TACATGG10950.031.3291342
GATACAT600.004144734731.3291322
ACCCTTG600.004144734731.3291327
ACATCCC452.5850302E-531.32913246-47
TGCAAAA300.00415507431.32913278-79