FastQCFastQC Report
Sun 14 Apr 2019
SRR6576177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340683
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5840.17142035264454053No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3960.11623708843705145No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3800.11154064041939281No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3660.10743124840394148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.10097363237966087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9750.049.168461
GTCCTAA555.2757263E-542.7266041
ATCAACG12050.039.783613
TCAACGC12150.039.4561774
AACGCAG12250.039.1340836
CAACGCA12450.038.8829275
TATCAAC14500.034.033952
ACGCAGA14250.033.97147
CGCAGAG14300.033.5239528
TACATGG21250.032.5124362
GTACATG21200.032.367421
ATAGCAC753.2710427E-431.3328423
ACATGGG22050.030.6933983
GCAGAGT16550.028.9663189
CATGGGA10900.027.1647154
GTAGATT700.00882812826.8567246
CATGGGG7750.026.6834554
ATGGGAG4750.024.7364565
ATGGGAT3300.022.7875235
TACGCTA3100.022.7415819
Produced by FastQC (version 0.11.2)