FastQCFastQC Report
Sun 14 Apr 2019
SRR6576177_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576177_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340683
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6340.1860967526997238No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4210.1235752884646431No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.12152059245691743No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4030.11829178444477711No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3710.10889888840945983No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3670.10772477640504516No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3450.10126716038076453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG22500.036.4411281
TACATGG22600.036.391992
ACATGGG23450.034.4716223
GTATCAA9900.033.7946741
GTCTATA600.004104424331.4147641
ATGGGAG4300.029.5101535
CATGGGA13100.029.4183774
ATGGGAT3800.027.2091675
CATGGGG8150.027.1029424
AACGGTA350.00883569626.855854-55
CATGGGT5150.026.4646974
AACGCAG12750.026.171246
CGCCGGT909.5066614E-426.1098067
TCAACGC12900.025.5026024
GTATAAT1859.711584E-925.4714321
CAACGCA13150.025.0177615
ATCAACG12800.024.9675033
ACTAATG1000.001756661123.4988273
TGGGTAC3750.022.5588726
TACGCTA2756.002665E-1122.2170729
Produced by FastQC (version 0.11.2)