FastQCFastQC Report
Sun 14 Apr 2019
SRR6576178_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576178_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309665
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6450.2082896032809649No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.14208903169554196No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4050.13078649508339657No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3910.1262654804385384No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3790.12239032502866No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3390.10947314032906529No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3230.10430626644922739No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3170.10236868874428817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC952.0869265E-839.6246341
GTATCAA10800.038.7761881
ACTCACG500.001695877337.619078
GTACATG21100.035.9039571
TACATGG21350.035.2232672
CATGGGT3900.034.9438554
ATCAACG12000.034.853483
ACATGGG22150.033.9456143
TCAACGC12400.033.729184
AACGCAG12150.033.649646
CAACGCA12800.032.675145
GGTACCT1950.031.3492248
GTATACT600.00415916231.3289744
GGGTACC2150.030.6003937
TATCAAC14950.028.6093352
ATGGGTA2650.028.373415
CGCAGAG14600.028.0210538
ACGCAGA14750.027.7181767
CATGGGA13150.027.5170824
CATGGGG7550.027.3869154
Produced by FastQC (version 0.11.2)