FastQCFastQC Report
Sun 14 Apr 2019
SRR6576179_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576179_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295042
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5560.18844774642254322No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.12981202676229148No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3520.1193050480948475No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3220.10913700422312755No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2990.10134150392147559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8150.041.636871
GTACATG20800.037.613871
TACATGG21250.036.723712
CATGGGT5650.036.6101534
ACATGGG21600.035.9110033
GTATTGG801.1339536E-535.3479731
CTAGACC550.002710703334.189653
CATGGGA10900.033.640734
ATGGGTG2950.033.465295
ATGGGAG3950.030.9437965
ATGGGTA3050.030.8267335
GGTACCT2450.030.6905028
AGTACAT12500.030.4629762
AACGCAG11150.030.3567286
CAACGCA11450.029.9719315
TCAACGC11500.029.8416184
ATCAACG11600.029.5843623
GAGTACA12650.029.433361
GGGTACC2550.027.6440167
ATTATAC1205.813534E-627.4229473
Produced by FastQC (version 0.11.2)