FastQCFastQC Report
Sun 14 Apr 2019
SRR6576180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269832
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5730.21235435382015475No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3820.1415695692134365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.1411989682469092No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3290.12192771798748851No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3050.11303329479083282No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2890.10710367932639567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA250.00523755256.3840984
GTACTAG502.9918256E-547.0303271
GTACATG21750.044.75991
TACATGG21950.044.1378022
GTATCAA9150.043.6893731
GTCTAGA555.2522708E-542.754841
ACATGGG22450.042.69623
ATGGGAG3800.038.3312955
CATGGGA11750.037.58944
GACCGTC500.001702075737.5893977
CATGGGG7350.035.7994274
ATCAACG11350.034.360353
ATGGGAT2900.034.0248875
AACGCAG11350.033.9463736
TCAACGC11600.033.6198274
CAACGCA11800.033.4481935
CATGGGC3350.032.259564
GTATAGA1052.0472926E-631.3535521
ATGGGCG600.004161129231.32455
ACGCAGA12750.030.2189317
Produced by FastQC (version 0.11.2)