FastQCFastQC Report
Sun 14 Apr 2019
SRR6576180_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576180_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269832
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5150.19085949776157018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.15861721367369325No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3520.1304515402176169No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3120.11562750155652406No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3120.11562750155652406No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3090.1145156986569421No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2880.10673307835986835No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2740.10154466482848587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9400.041.538991
TACATGG21150.038.437782
GTACATG21450.037.7230531
ACATGGG21450.037.0238763
CATGGGT6050.034.1758884
AACGCAG11400.033.3889436
GTGTAAG851.7386175E-533.2076381
ATGGGAC2150.032.7850075
ATCAACG11800.032.2571143
TCAACGC11900.031.9860484
CAACGCA12100.031.4573545
GTCTAGT600.004136329531.362771
GTACTAG600.004136329531.362771
GGTTAGC600.00414384931.3511378
ATGGGTG3000.029.7615035
TATCAAC13700.029.4985312
ACGCAGA12900.029.1422317
CATGGGA11350.028.9817564
TGCACCG650.006151150428.9180585
ATGGGAG4400.028.8359055