FastQCFastQC Report
Sun 14 Apr 2019
SRR6576181_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576181_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635354
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.16006824541908918No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9190.14464377339247098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17700.041.8258131
ATCAACG20500.035.5323333
CAACGCA21700.033.7839745
TAGACGT702.185443E-433.567415
TACATGG33750.033.4313772
TCAACGC21850.033.336974
GTACATG34450.033.260981
GTACCGT851.754989E-533.17256
AACGCAG22750.032.2247126
CGCACCG753.2762048E-431.329585
ACATGGG36500.030.5141533
TATCAAC24500.029.9347292
ATGGGTA5100.029.4866665
TACGCTC804.7774494E-429.37610694
GGTATCA9450.028.9410421
CATGGGT8150.028.8309044
AAGACCG1005.3241787E-528.1966255
GCACCGT2002.7284841E-1128.1966256
ACGCAGA26450.028.0722527
CGCAGAG27050.027.449588
Produced by FastQC (version 0.11.2)